diff --git a/gnu/local.mk b/gnu/local.mk
index 8366ffcc19..516742d767 100644
--- a/gnu/local.mk
+++ b/gnu/local.mk
@@ -1760,6 +1760,7 @@ dist_patch_DATA =						\
   %D%/packages/patches/libphonenumber-reproducible-build.patch	\
   %D%/packages/patches/libqalculate-3.8.0-libcurl-ssl-fix.patch	\
   %D%/packages/patches/libquicktime-ffmpeg.patch 		\
+  %D%/packages/patches/libsecret-fix-test-paths.patch		\
   %D%/packages/patches/libsepol-versioned-docbook.patch		\
   %D%/packages/patches/libtar-CVE-2013-4420.patch 		\
   %D%/packages/patches/libtar-CVE-2021-33643-CVE-2021-33644.patch	\
diff --git a/gnu/packages/gnome.scm b/gnu/packages/gnome.scm
index a7d6c4d9f9..979e7f2447 100644
--- a/gnu/packages/gnome.scm
+++ b/gnu/packages/gnome.scm
@@ -5291,6 +5291,7 @@ and the GLib main loop, to integrate well with GNOME applications.")
                     "mirror://gnome/sources/libsecret/"
                     (version-major+minor version) "/"
                     "libsecret-" version ".tar.xz"))
+              (patches (search-patches "libsecret-fix-test-paths.patch"))
               (sha256
                (base32
                 "081bj59ws08kb261cd1w1mkdkhfbzsjbbkkrm6wllvdyhgbhhg8n"))))
diff --git a/gnu/packages/patches/libsecret-fix-test-paths.patch b/gnu/packages/patches/libsecret-fix-test-paths.patch
new file mode 100644
index 0000000000..e4cdbf92a6
--- /dev/null
+++ b/gnu/packages/patches/libsecret-fix-test-paths.patch
@@ -0,0 +1,37 @@
+From 208989323211c756dff690115e5cbde5ef7491ce Mon Sep 17 00:00:00 2001
+From: Bobby Rong <rjl931189261@126.com>
+Date: Sun, 10 Mar 2024 10:30:48 +0800
+Subject: [PATCH] meson: Use env.prepend() for test environment setup
+
+On NixOS packages are installed in separate prefixes.
+Starting from GLib / gobject-introspection 2.80, GLib introspection data
+is provided by GLib itself instead of gobject-introspection. This causes
+tests failures on NixOS because env.set() resets the environment and GLib
+is missing from GI_TYPELIB_PATH:
+
+gi.RepositoryError: Typelib file for namespace 'Gio', version '2.0' not found
+
+See also:
+https://gitlab.gnome.org/GNOME/gtk/-/commit/29e6cc58088ef37bfde364965cdebd2f7402f315
+---
+ libsecret/meson.build | 4 ++--
+ 1 file changed, 2 insertions(+), 2 deletions(-)
+
+diff --git a/libsecret/meson.build b/libsecret/meson.build
+index 85ad68f..ea417ba 100644
+--- a/libsecret/meson.build
++++ b/libsecret/meson.build
+@@ -257,8 +257,8 @@ if get_option('introspection')
+   # env to be used in tests that use the typelib,
+   # to make sure they find the one for MockService
+   test_typelib_env = environment()
+-  test_typelib_env.set('GI_TYPELIB_PATH', meson.current_build_dir())
+-  test_typelib_env.set('LD_LIBRARY_PATH', meson.current_build_dir())
++  test_typelib_env.prepend('GI_TYPELIB_PATH', meson.current_build_dir())
++  test_typelib_env.prepend('LD_LIBRARY_PATH', meson.current_build_dir())
+ 
+   # Python Tests
+   pytest_names = [
+-- 
+GitLab
+